Hi all,
I received some fastq files from a PE HiSeq and when I tried to isolate N reads using the "fastq_illumina_reads -N" I got the following error:
Input error: file 'STDIN' line 1: Expecting Illumina-CASAVA1.8 ID line structure (@<instrument>:<run number>:<flowcell ID>:<lane>:<tile>:<x-pos>:<y-pos> <read>:<is filtered>:<control number>:<index sequence>) - got '@HS34_23148:4:1313:16347:42480/1' (Can't extract 'Tile’)
The header of the file shows:
@HS34_23148:4:2106:12625:73859/2
CACCAGCTGAGAGAGATGCTCGCCGTTGACTGACGAACTGAATTCCCAGTTCACGGCGGTATGGAATACCGTCGT
+
BBBBBFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@HS34_23148:4:1114:12855:77248/2
CTCGCCGTTGACTGACGAACTGAATTCCCAGTTCACGGCGGTATGGAATACCGTCGTCGCAGAGCTCAACGGTGA
+
BBBBBFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFF
@HS34_23148:4:1311:2663:94744/2
CTCGACAGATATTGATTGTCGTCACCGTTGAGCTCTGCGACGACGGTATTCCATACCGCCGTGAACTGGGAATTC
Does anyone already get the same problem with the tile coordinates?
(I could ask the facility who sent me the files an explanation but I'm not sure if the problem come from me or not)
Thank you
I received some fastq files from a PE HiSeq and when I tried to isolate N reads using the "fastq_illumina_reads -N" I got the following error:
Input error: file 'STDIN' line 1: Expecting Illumina-CASAVA1.8 ID line structure (@<instrument>:<run number>:<flowcell ID>:<lane>:<tile>:<x-pos>:<y-pos> <read>:<is filtered>:<control number>:<index sequence>) - got '@HS34_23148:4:1313:16347:42480/1' (Can't extract 'Tile’)
The header of the file shows:
@HS34_23148:4:2106:12625:73859/2
CACCAGCTGAGAGAGATGCTCGCCGTTGACTGACGAACTGAATTCCCAGTTCACGGCGGTATGGAATACCGTCGT
+
BBBBBFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
@HS34_23148:4:1114:12855:77248/2
CTCGCCGTTGACTGACGAACTGAATTCCCAGTTCACGGCGGTATGGAATACCGTCGTCGCAGAGCTCAACGGTGA
+
BBBBBFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFF
@HS34_23148:4:1311:2663:94744/2
CTCGACAGATATTGATTGTCGTCACCGTTGAGCTCTGCGACGACGGTATTCCATACCGCCGTGAACTGGGAATTC
Does anyone already get the same problem with the tile coordinates?
(I could ask the facility who sent me the files an explanation but I'm not sure if the problem come from me or not)
Thank you
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