Hi,
I don't know what's going on; after running RS_Resequencing.1 from SMART Portal (because the protocol BAM_Resequencing_Beta.1 did't work), I get a mapping report of ca. 90K reads mapped. But when I select to download the aligned reads BAM file (down in Resequencing panel) I get a lousy file:
I guess there is something I missed, but what?
PS. the H5 aligned reads file looks bigger, at least in size.
PS2. I set Max. divergence 30%
Thanks
I don't know what's going on; after running RS_Resequencing.1 from SMART Portal (because the protocol BAM_Resequencing_Beta.1 did't work), I get a mapping report of ca. 90K reads mapped. But when I select to download the aligned reads BAM file (down in Resequencing panel) I get a lousy file:
Code:
samtools flagstat pacbio.bam 418 in total 0 QC failure 0 duplicates 418 mapped (100.00%)
PS. the H5 aligned reads file looks bigger, at least in size.
PS2. I set Max. divergence 30%
Thanks
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