I'm prepping a small RNA library with the TruSeq Small RNA library kit. I typically use the RA5 adapter provided in the kit. For my current experiment, I need a second 5' adapter that I can distinguish from RA5.
I want to ligate one 5' adapter onto one RNA class and a second distinct 5' adapter onto a different RNA class. Each adapter needs to have a region of unique sequence so they can be distinguished. Any recommendations for barcoded adapters like this? Or thoughts on how they should be designed? Thanks!
I want to ligate one 5' adapter onto one RNA class and a second distinct 5' adapter onto a different RNA class. Each adapter needs to have a region of unique sequence so they can be distinguished. Any recommendations for barcoded adapters like this? Or thoughts on how they should be designed? Thanks!
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