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I do have a set of data containing both Exome Seq and RNA Seq. It will be interesting to see how they correlate in terms of calling. Specifically, it will be interested to see how mark duplication will affect the concordance between the RNA samples from the DNA samples. However, I do have a question: is it valid to use tools like GATK to perform snp calling on RNA Samples? To my knowledge, it seems like GATK might contain certain prior specifically designed for exome sequencing (or whole genome sequencing). Wouldn't that also affect the call concordance in the final data?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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