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Old 03-28-2010, 11:57 AM   #1
fisher
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Question Can you really see miRNAs on gels before PCR amplification

I am new to miRNAseq. We plan to do miRNAseq using small RNA prep V1.0 and I am wondering if we could really see miRNAs on gel after each ligation?

Thanks a lot.
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Old 07-21-2010, 09:24 AM   #2
kwaraska
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Default miRNA

I have never been able to see them. I just do a blind cut and pray a lot!
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Old 07-21-2010, 09:44 AM   #3
fisher
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Thank you!

I finally cut it blindly too. But it worked!



Quote:
Originally Posted by kwaraska View Post
I have never been able to see them. I just do a blind cut and pray a lot!
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Old 07-27-2010, 07:37 PM   #4
link1
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if you use an adenylated 3' adapter you don't have to perform the small RNA gels and cuts. the adapter specifically will bind to your smalls.
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