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Old 04-25-2011, 11:05 AM   #1
rdt23
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Location: MA

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Default Obtaining 500bp DNA fragments from Gels

Hi Everyone,
We currently only use e-gels in our lab, and I need to obtain equipment for being able to cut out the 500bp DNA fragments after running electrophoresis. I am aware that this is "old school", but have never done it before! Any suggestions and help would be much appreciated.
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Old 04-25-2011, 11:14 AM   #2
ECO
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Get the E-gel cracker and cut the band out of the Egel. Dangerous as hell, but it works. http://products.invitrogen.com/ivgn/...search-product

Alternatively, I really like Bio-rad ReadyAgarose Gels, they are fully compatible with their hand-cast gel rigs, so if you end up having to pour your own you'll already have the equipment:

https://www.bio-rad.com/prd/en/US/ad...e-c2bb071ca7d2
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Old 04-25-2011, 11:20 AM   #3
rdt23
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Thanks very much! Think I might try that e-gel cracker first...
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