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04-25-2011, 11:05 AM
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#1 |
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Junior Member
Location: MA Join Date: Mar 2011
Posts: 3
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Obtaining 500bp DNA fragments from Gels
Hi Everyone,
We currently only use e-gels in our lab, and I need to obtain equipment for being able to cut out the 500bp DNA fragments after running electrophoresis. I am aware that this is "old school", but have never done it before! Any suggestions and help would be much appreciated. |
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04-25-2011, 11:14 AM
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#2 |
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--Site Admin--
Location: SF Bay Area, CA, USA Join Date: Oct 2007
Posts: 1,358
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Get the E-gel cracker and cut the band out of the Egel. Dangerous as hell, but it works. http://products.invitrogen.com/ivgn/...search-product
Alternatively, I really like Bio-rad ReadyAgarose Gels, they are fully compatible with their hand-cast gel rigs, so if you end up having to pour your own you'll already have the equipment: https://www.bio-rad.com/prd/en/US/ad...e-c2bb071ca7d2 |
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04-25-2011, 11:20 AM
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#3 |
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Junior Member
Location: MA Join Date: Mar 2011
Posts: 3
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Thanks very much! Think I might try that e-gel cracker first...
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