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Old 03-18-2010, 07:28 AM   #1
basager
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Default Ovation RNA-seq system

Have anyone tried the Ovation RNA-seq system from NuGen? I have small amounts of input RNA and consider to use this system to have enough starting material for RNA-seq. Any experience?

Last edited by basager; 03-18-2010 at 08:39 AM.
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Old 06-10-2010, 09:32 AM   #2
kainsteven
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Default Users of the Ovation RNA-Seq System

We have several conference posters and webinars posted on our web site to illustrate studies with low inputs of total RNA using the Ovation RNA-Seq System: http://www.nugeninc.com/nugen/index....na-seq-system/

Best,
Steve

Last edited by kainsteven; 06-11-2010 at 12:57 PM.
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Old 06-23-2010, 10:50 AM   #3
ShannonJ
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I love the NuGen kits. We routinely use them to generate random cDNA from viral RNA samples. If you follow with the Exon Module the products can be used for beautiful Illumina libraries.
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Old 06-25-2010, 09:57 AM   #4
kainsteven
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Default Ovation RNA-Seq, 3'-DGE and Encore NGS Library Systems

It is no longer necessary to use the exon module to produce the double-stranded cDNA. We have packaged the complete solution for producing a dscDNA starting with total RNA in either an RNA-Seq or 3'-DGE experiment. Each of these workflows integrate directly with our Encore NGS Library Systems for the Illumina platform. Workflow time from total RNA to cBot is about 9 hours.

Steve
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Old 07-27-2010, 08:38 AM   #5
paul z
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I'm having a difficult time understanding how, in the absence of a polyA selection or a ribosomal reduction method, and through the use of a mix of oligo dT and random primers, NuGEN is able to achieve such purportedly low % mapping to rRNA (~ 3-3.5%) -- are the random primers not_so_random, i.e., do they select against rRNA sequences?

Anyone try NuGEN in house and obtain similar metrics as reported in NuGEN's product literature? (http://www.nugeninc.com/tasks/sites/...ov_rna_seq.pdf)

Curious,
-Paul
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Old 07-27-2010, 01:21 PM   #6
ShannonJ
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We've used them to sequence viruses along with host "background" sequence. With one channel of SE 76bp Illumina we get about 90% coverage of the viral genome, and little host rRNA.
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