Hello,
I'm running cuffdiff for DE.
I'm running with -c parameter as 10. I'm gettig test OK, while the raw counts at that locus are lower than 10.
I have two conditions: con_1 have 2 replicates, and con_2 have one replicate.
The raw counts for con_1 are:
0 for the first replicate
3 for the second replicate
The raw count for con_2 is: 2
I can't understand why the test is OK. isn't it should be NOTEST in that case?
one more question: how does cuffdiff treat replicates in a condition to set the status of the test? does it sum all the raw counts and compare the sum to the -c parameter? Does it do something alse?
Thanks,
Maya
I'm running cuffdiff for DE.
I'm running with -c parameter as 10. I'm gettig test OK, while the raw counts at that locus are lower than 10.
I have two conditions: con_1 have 2 replicates, and con_2 have one replicate.
The raw counts for con_1 are:
0 for the first replicate
3 for the second replicate
The raw count for con_2 is: 2
I can't understand why the test is OK. isn't it should be NOTEST in that case?
one more question: how does cuffdiff treat replicates in a condition to set the status of the test? does it sum all the raw counts and compare the sum to the -c parameter? Does it do something alse?
Thanks,
Maya