Hi,
I am about to prepare some libraries with precious tissue for RNA-seq. I'd like to protect them as much as I can from RNA degradation, but I'm not positive that all RNAse inhibitors out there are compatible with downstream applications such as sequencing. Most of them state being safe for cDNA synthesis, but none of them mention sequencing per se, and I dont't know if there may be some slight details in the workflow that will not work as well if this is added.
I am considering Roche's Protector and Life Tech's RNAsecure.
Have you used RNAse inhibitors with success in making your libraries?
Thanks!
C
I am about to prepare some libraries with precious tissue for RNA-seq. I'd like to protect them as much as I can from RNA degradation, but I'm not positive that all RNAse inhibitors out there are compatible with downstream applications such as sequencing. Most of them state being safe for cDNA synthesis, but none of them mention sequencing per se, and I dont't know if there may be some slight details in the workflow that will not work as well if this is added.
I am considering Roche's Protector and Life Tech's RNAsecure.
Have you used RNAse inhibitors with success in making your libraries?
Thanks!
C
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