Getting RIN score from Agilent's mRNA program?

Transcript · 07-31-2015, 03:10 PM

Hello all (1st post here!),

I recently ran some RNA extraction samples on Agilent's Bioanalyzer for QC check. However, I realized afterward that Agilent doesn't calculate RIN scores for its mRNA protocol, only for the Total RNA protocol.

The data collected is the same, and the program ought to be able to get a RIN score from the trace (electropherogram). Is there a way to voodoo the program into giving me a RIN score on the mRNA protocol?

(Yes, I know that the most accurate method is to examine the traces and make a personal evaluation of the quality. But it would be great to have a summary RIN score for each sample as well.)

nucacidhunter · 08-01-2015, 01:59 AM

There are some information on RIN calculation in this application note: http://www.chem.agilent.com/Library/...989-1165EN.pdf

pmiguel · 08-03-2015, 09:24 AM

Briefly, no it isn't possible because mRNA doesn't contain rRNA peaks and these are the primary focus of a RIN score.

--
Phillip

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07-31-2015, 03:10 PM	#1
Transcript Junior Member Location: California, US Join Date: Jul 2015 Posts: 1	Getting RIN score from Agilent's mRNA program? Hello all (1st post here!), I recently ran some RNA extraction samples on Agilent's Bioanalyzer for QC check. However, I realized afterward that Agilent doesn't calculate RIN scores for its mRNA protocol, only for the Total RNA protocol. The data collected is the same, and the program ought to be able to get a RIN score from the trace (electropherogram). Is there a way to voodoo the program into giving me a RIN score on the mRNA protocol? (Yes, I know that the most accurate method is to examine the traces and make a personal evaluation of the quality. But it would be great to have a summary RIN score for each sample as well.)

08-01-2015, 01:59 AM	#2
nucacidhunter Jafar Jabbari Location: Melbourne Join Date: Jan 2013 Posts: 1,238	There are some information on RIN calculation in this application note: http://www.chem.agilent.com/Library/...989-1165EN.pdf Last edited by nucacidhunter; 08-01-2015 at 02:02 AM.

08-03-2015, 09:24 AM	#3
pmiguel Senior Member Location: Purdue University, West Lafayette, Indiana Join Date: Aug 2008 Posts: 2,317	Briefly, no it isn't possible because mRNA doesn't contain rRNA peaks and these are the primary focus of a RIN score. -- Phillip