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Old 04-22-2011, 08:20 AM   #1
Retro
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Location: Pennsylvania

Join Date: Apr 2011
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Default NEBNext Quick DNA Sample Prep - buying individual enzymes separately

NEB is selling 2 kits to prepare 454 libraries using dA-tailed ligation:

1. NEBNext Quick DNA Sample Prep Master Mix Set 2
2. NEBNext Quick DNA Sample Prep Reagent Set 2

In the first kit they give the actual enzyme concentrations in the mix, except for the Quick ligase. To decrease the price in preparing 20 libraries, I want to order the NEB enzymes separately. Does anyone have experience in doing it and how much of the Quick ligase (NEB cat. n. M2200S) you use?

Thanks.
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