repeating chipseq or chipseq with another antibody

hawainpanda · 07-12-2011, 07:15 PM

I was wondering which was the preferred method?
Would people prefer performing chipseq with two diff antibodies, or repeating chipseq with same antibody?

mziemann · 03-12-2015, 04:34 AM

Not sure what you mean. Once an antibody is validated as specific, it should be fine to use. The more biological and technical replicates you perform the better.

kerplunk412 · 03-12-2015, 04:56 PM

As a reviewer, ChIP-Seq with a different antibody would definitely add strength to a manuscript. Biological triplicates with both antibodies would be ideal. I think people sometimes put too much faith in antibody specificity, so I think data generated with two different antibodies is much more convincing. Also, an IP-Western with an IP done with one antibody and the Western done with another is always a strong piece of data to add to a manuscript, in my opinion.

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07-12-2011, 07:15 PM	#1
hawainpanda Junior Member Location: texas Join Date: Jul 2011 Posts: 2	repeating chipseq or chipseq with another antibody I was wondering which was the preferred method? Would people prefer performing chipseq with two diff antibodies, or repeating chipseq with same antibody?

03-12-2015, 04:34 AM	#2
mziemann Member Location: Australia Join Date: Aug 2010 Posts: 10	Not sure what you mean. Once an antibody is validated as specific, it should be fine to use. The more biological and technical replicates you perform the better.

03-12-2015, 04:56 PM	#3
kerplunk412 Senior Member Location: Bioo Scientific, Austin, TX, USA Join Date: Jun 2012 Posts: 119	As a reviewer, ChIP-Seq with a different antibody would definitely add strength to a manuscript. Biological triplicates with both antibodies would be ideal. I think people sometimes put too much faith in antibody specificity, so I think data generated with two different antibodies is much more convincing. Also, an IP-Western with an IP done with one antibody and the Western done with another is always a strong piece of data to add to a manuscript, in my opinion.