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11-14-2011, 03:37 AM
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#1 |
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still a novice
Location: Cardiff Join Date: Jul 2010
Posts: 34
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TruSeq library size
Hi all,
I'm setting up a library prep using TruSeq DNA sample enrichment kits, I've sheared the DNA using Covaris and have run the product on a bioanalyzer. My library peaks seem to be around the 55-0600bp mark whereas previously I would get them nearer 300bp. I'm planning to sequence on an illumiina GA11x with 110bp read length, does anyone one have an idea if these fragments need to be sheared again to make them smaller or are they OK? Thanks in advance for any help - H |
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11-14-2011, 03:46 AM
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Senior Member
Location: Birmingham, UK Join Date: Jul 2009
Posts: 356
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Should be OK. And as a bonus, if you sequence in both directions you'll get a nice long insert which should help with mapping or assembly.
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11-14-2011, 03:56 AM
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#3 | |
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still a novice
Location: Cardiff Join Date: Jul 2010
Posts: 34
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Quote:
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