Bioruptor shearing of PCR product

bernieball · 04-06-2012, 11:25 AM

We are pooling multiple PCR products that range in size from 700 to 2000 bp and would like to fragment them to approx. 450-500 bp before tagging them with barcodes.

Has anyone worked with PCR products vs. genomic DNA in a similar manner?

We will be using a Bioruptor UCD-200.

Thanks for your help!

jchung · 11-25-2013, 07:37 AM

Bernie,

I'm curious to know how your shearing worked for those mixed amplicon sizes. We are trying to develop a shearing protocol as well for amplicon pools of 300bp - 2kb.

Thanks!

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04-06-2012, 11:25 AM	#1
bernieball Junior Member Location: North Carolina Join Date: Apr 2012 Posts: 1	Bioruptor shearing of PCR product We are pooling multiple PCR products that range in size from 700 to 2000 bp and would like to fragment them to approx. 450-500 bp before tagging them with barcodes. Has anyone worked with PCR products vs. genomic DNA in a similar manner? We will be using a Bioruptor UCD-200. Thanks for your help!

11-25-2013, 07:37 AM	#2
jchung Junior Member Location: Washington, D.C. Join Date: Nov 2013 Posts: 1	Bernie, I'm curious to know how your shearing worked for those mixed amplicon sizes. We are trying to develop a shearing protocol as well for amplicon pools of 300bp - 2kb. Thanks!