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Thread | Thread Starter | Forum | Replies | Last Post |
Raw read counts for RNAseq | biofreak | Introductions | 13 | 01-16-2013 06:28 AM |
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How can one get raw read counts from RPKM values | gen2prot | General | 6 | 06-24-2011 12:08 PM |
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How to convert cufflinks output to raw counts | jebe | RNA Sequencing | 0 | 01-26-2010 12:29 PM |
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#1 |
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Location: USA Join Date: Jun 2011
Posts: 44
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How do I obtain from the SAM/BAM file raw read counts per exon and not the gene?
thanks. |
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#2 |
Senior Member
Location: Kansas City Join Date: Mar 2008
Posts: 197
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If you make a bed file of exons, you can use bedtools coverageBed to tally counts per exon.
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#3 |
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Location: USA Join Date: Jun 2011
Posts: 44
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thanks. How do I obtain exon.bed for rat?
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#4 |
Senior Member
Location: Kansas City Join Date: Mar 2008
Posts: 197
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I'm guessing you could get the info from ensembl biomart. (structures, exon)
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