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Old 08-02-2012, 07:13 AM   #1
Location: New York

Join Date: Mar 2011
Posts: 26
Default low library concentration

Hi Friends

I am using TruSeq whole RNA sequencing kit. I used 50 ng input of earlier purified mRNA and gave a 15 cycle. At the end, the libraries were dissolved in 30 microliter of RSB buffer. Out of 4 libraries, one of the library is 1/10 of others in terms of concentration of dsDNA. Should I be worried? I have not yet done any bioanalyzer analysis.

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