Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
==> magnetic bead separation magnet Zimbobo Ion Torrent 22 05-24-2020 10:22 AM
ampure purification of illumina libraries greigite Sample Prep / Library Generation 23 09-13-2016 06:15 AM
Automated AMPure Purification Anthony.287 454 Pyrosequencing 1 08-21-2012 12:59 PM
Agencourt Ampure purification kit - exp date ntremblay Sample Prep / Library Generation 3 03-06-2012 04:41 AM
alternatives to sera-mag bead purification peromhc Sample Prep / Library Generation 1 05-12-2010 10:03 AM

Thread Tools
Old 11-18-2012, 08:42 AM   #1
Junior Member
Location: Bavaria

Join Date: Nov 2012
Posts: 3
Default Ampure magnetic bead purification -- how does it work ?

Hi I'm new in using NGS especially the GS Junior.
I prepaired a library using self designed primers. After amplification there's a purification step by magnetic beads. I understand the basics but not every reaction is clear to me. My main questions are: Why do they say that its equal using 10l library or fewer and add some water. Doesn't this change my purification result?
In the patent of this technique the salt concentration is mentioned to influence the results. Why and what does salt do. If I add water, my salt concentration is reduced isn't it? Have to explaine this method in my thesis.
Could you please try to explain the chemical background to me.

Thanking you in anticipation!
Regards Christine90
Christine90 is offline   Reply With Quote
Old 11-18-2012, 09:24 AM   #2
--Site Admin--
Location: SF Bay Area, CA, USA

Join Date: Oct 2007
Posts: 1,358

There are plenty of threads about the mechanism...please
search, especially if it's for your thesis!
ECO is offline   Reply With Quote
Old 11-19-2012, 05:16 AM   #3
Senior Member
Location: London

Join Date: Jun 2009
Posts: 298
TonyBrooks is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 02:17 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO