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Old 01-08-2013, 11:50 AM   #1
dr7ski
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Location: Colorado

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Default Illumina ITS fusion primers for fungi

Anyone preparing ITS amplicon libraries for use on a MiSeq platform? Care to share any of your primer designs? Need to start examining fungal communities and trying not to reinvent the wheel.

Thanks!
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Old 04-17-2013, 02:30 PM   #2
jennomics
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I'm wondering the same thing. What did you end up doing? Any suggestions?
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Old 04-25-2013, 08:28 AM   #3
EricP
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Hi There,
I have the protocol for ITS Fungal in HiSeq working. New forward primer, better coverage than the previous described, we are preparing the paper now.
Cheers

Eric
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Old 06-17-2013, 08:36 AM   #4
chapadosj
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I am currently designing Illumina fusion primers but am getting a bit worried. I don't want to get stuck buying 96 unique really long fusion primers (I want dual indexing). Do you know of anyone using complete fusion primers (adaptor+seq primer+index+PCR primer) VERSUS ligating adaptor+seq primer+index to standard PCR product after amplification?
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Old 12-02-2013, 08:17 AM   #5
fungi122
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Hi Eric, would like to check if your paper already published. If so, could you please send me the link and share the protocol &primers? Thank you so much!!
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