Illumina ITS fusion primers for fungi

dr7ski · 01-08-2013, 11:50 AM

Anyone preparing ITS amplicon libraries for use on a MiSeq platform? Care to share any of your primer designs? Need to start examining fungal communities and trying not to reinvent the wheel.

Thanks!

jennomics · 04-17-2013, 02:30 PM

I'm wondering the same thing. What did you end up doing? Any suggestions?

EricP · 04-25-2013, 08:28 AM

Hi There,
I have the protocol for ITS Fungal in HiSeq working. New forward primer, better coverage than the previous described, we are preparing the paper now.
Cheers

Eric

chapadosj · 06-17-2013, 08:36 AM

I am currently designing Illumina fusion primers but am getting a bit worried. I don't want to get stuck buying 96 unique really long fusion primers (I want dual indexing). Do you know of anyone using complete fusion primers (adaptor+seq primer+index+PCR primer) VERSUS ligating adaptor+seq primer+index to standard PCR product after amplification?

fungi122 · 12-02-2013, 08:17 AM

Hi Eric, would like to check if your paper already published. If so, could you please send me the link and share the protocol &primers? Thank you so much!!

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01-08-2013, 11:50 AM	#1
dr7ski Junior Member Location: Colorado Join Date: Jul 2012 Posts: 1	Illumina ITS fusion primers for fungi Anyone preparing ITS amplicon libraries for use on a MiSeq platform? Care to share any of your primer designs? Need to start examining fungal communities and trying not to reinvent the wheel. Thanks!

04-17-2013, 02:30 PM	#2
jennomics Junior Member Location: Davis, CA Join Date: Apr 2013 Posts: 2	I'm wondering the same thing. What did you end up doing? Any suggestions?

04-25-2013, 08:28 AM	#3
EricP Junior Member Location: Switzerland Join Date: Jan 2013 Posts: 2	Hi There, I have the protocol for ITS Fungal in HiSeq working. New forward primer, better coverage than the previous described, we are preparing the paper now. Cheers Eric

06-17-2013, 08:36 AM	#4
chapadosj Junior Member Location: Ottawa ON Canada Join Date: Jun 2013 Posts: 2	I am currently designing Illumina fusion primers but am getting a bit worried. I don't want to get stuck buying 96 unique really long fusion primers (I want dual indexing). Do you know of anyone using complete fusion primers (adaptor+seq primer+index+PCR primer) VERSUS ligating adaptor+seq primer+index to standard PCR product after amplification?

12-02-2013, 08:17 AM	#5
fungi122 Junior Member Location: USA Join Date: Dec 2013 Posts: 1	Hi Eric, would like to check if your paper already published. If so, could you please send me the link and share the protocol &primers? Thank you so much!!