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Old 04-15-2013, 02:47 AM   #1
aleferna
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Location: sweden

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Default Miseq ReagentValve

13-04-13 13:58:33.872 +01 000000017 INF: Selector valve "ReagentValve" timed out on attempt #1: "ReagentValve: '+08\r' command timed out waiting 60000/60000 ms for any of "'*','\r'"; received ''


Hi, we are running the Maintenance wash in the MiSeq with the latest MCS 2.2 and we get this error message at the very end of the wash. We've reproduced it 5 times (restarted software, turned power off for 10 min, etc, etc) now and Illumina support says that it is a broken valve and they will have to replace it, but we really need to run a sample and the replacement but I fear it will take too long.

Has anybody had this problem? Any quick fixes that could save us ?
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Old 04-15-2013, 10:19 AM   #2
kcchan
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There's nothing you can do if the valve is dead, it's a hardware problem and the valve needs replacing. Has this MiSeq been upgraded to v2? I'm surprised to hear that there are still valve issues even with the redesigned valve.
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Old 04-15-2013, 10:35 AM   #3
aleferna
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yeap, but I'm not sure if we ever did get the valve replaced.. I remember the tech mentioned something about replacing it, but I'm not sure if he finally did replace it.
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