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06-19-2013, 11:30 AM
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#1 |
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Senior Member
Location: Oregon Join Date: Apr 2011
Posts: 205
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bacterial genome re-sequencing
I have a customer that wants to re-sequence 24 different bacterial genomes. What would be a reasonable level of multiplexing on GS Jr assuming an average genome size of 5-6 Mb and optimal (from your experience) for resequencing coverage depth?
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07-11-2013, 07:53 AM
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#2 |
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Junior Member
Location: Amsterdam Join Date: Dec 2009
Posts: 4
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A coverage depth of 15-20x in our hands on a FLX+ is more then enough for a 2Mb bacterial genome. More coverage didn't lead to less contigs.
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