Reusing/Recycling Probes?

naji · 09-21-2016, 10:33 AM

Hi all,

Just wondering if anyone has had any luck with successfully, or trying to, recycle/reuse DNA probes? Any advice, ideas, or insights would help.

Thanks!

GenoMax · 09-21-2016, 10:36 AM

Why would you want to do that? This (to a non-bench scientist) sounds like a bad idea.

naji · 09-21-2016, 10:59 AM

I figure to save money on probes, but also, if this is something that can be done (relatively) "easily" and efficiently, it could allow for using recycled probes as a control variable for assay development?

nucacidhunter · 09-21-2016, 02:47 PM

Do you mean biotinylated sequence capture probes?

HESmith · 09-21-2016, 04:11 PM

Quote:

Originally Posted by GenoMax

Why would you want to do that? This (to a non-bench scientist) sounds like a bad idea.

This (to a bench scientist) also sounds like a bad idea. In theory, you can strip the sequence capture probes. In practice, you'll get contaminating sequences from previous captures.

BioDynami · 10-13-2016, 08:49 PM

Compared to the money you saved, you will get more trouble in terms of library contamination and possible bias of probe contents.

Xploration · 10-20-2016, 09:07 AM

What kind of probes are you asking about? I re-use DIG-labeled PCR product probes for Southern blots.

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09-21-2016, 10:33 AM	#1
naji Junior Member Location: San Francisco Bay Area Join Date: Sep 2016 Posts: 3	Reusing/Recycling Probes? Hi all, Just wondering if anyone has had any luck with successfully, or trying to, recycle/reuse DNA probes? Any advice, ideas, or insights would help. Thanks!

09-21-2016, 10:36 AM	#2
GenoMax Senior Member Location: East Coast USA Join Date: Feb 2008 Posts: 7,091	Why would you want to do that? This (to a non-bench scientist) sounds like a bad idea.

09-21-2016, 10:59 AM	#3
naji Junior Member Location: San Francisco Bay Area Join Date: Sep 2016 Posts: 3	I figure to save money on probes, but also, if this is something that can be done (relatively) "easily" and efficiently, it could allow for using recycled probes as a control variable for assay development?

09-21-2016, 02:47 PM	#4
nucacidhunter Jafar Jabbari Location: Melbourne Join Date: Jan 2013 Posts: 1,238	Do you mean biotinylated sequence capture probes?

10-13-2016, 08:49 PM	#6
BioDynami Registered Vendor Location: Alabama Join Date: Jul 2016 Posts: 23	Compared to the money you saved, you will get more trouble in terms of library contamination and possible bias of probe contents.

10-20-2016, 09:07 AM	#7
Xploration Junior Member Location: Michigan Join Date: Jun 2014 Posts: 1	What kind of probes are you asking about? I re-use DIG-labeled PCR product probes for Southern blots.