Dear all,
I have a raw data of re-sequencing of M. tuberculosis' genome. We run on GAIIx system with paired end (50 bp sequence run).
I try to assembly with Velvet programs with default kmer=31. We obtain config files with 2344 nodes (open with Genious program), we ligate all contigs by order and have a concatenated sequence with 4.559.459 bp.
This is the first time I work with this field, so I'm wondering this is a right process to have complete genome sequences? How to check SNP from this sequences?...
Thank for any suggestion!
I have a raw data of re-sequencing of M. tuberculosis' genome. We run on GAIIx system with paired end (50 bp sequence run).
I try to assembly with Velvet programs with default kmer=31. We obtain config files with 2344 nodes (open with Genious program), we ligate all contigs by order and have a concatenated sequence with 4.559.459 bp.
This is the first time I work with this field, so I'm wondering this is a right process to have complete genome sequences? How to check SNP from this sequences?...
Thank for any suggestion!
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