Hi all!
I've some questions about Oxford nanopore Minion technology. I had a deep insight into the community of nanopore but I was unable to find out the answers:
1. What does it mean the "bps" terms of the workflow protocols of Metrichor? Is it just a kind of Internet connection?
2. I've just run the Burn-in protocol. How can I compare the results in order to be sure that the sequencing process ran properly?
3. How should I proceed in order to re-use the flow cell? I mean, the flow cell will have a kind of contamination DNA, won't it?
4. Finally, is there a kind of amplification in the library construction that allows to assembly several reads in order to obtain a larger genomic sequence than those gathered by Minion (I mean larger than 16Kb, for instance).
Sorry if my questions annoyed anyone, but I am still as Illumina-thinker
Thank you for your help in advance.
I've some questions about Oxford nanopore Minion technology. I had a deep insight into the community of nanopore but I was unable to find out the answers:
1. What does it mean the "bps" terms of the workflow protocols of Metrichor? Is it just a kind of Internet connection?
2. I've just run the Burn-in protocol. How can I compare the results in order to be sure that the sequencing process ran properly?
3. How should I proceed in order to re-use the flow cell? I mean, the flow cell will have a kind of contamination DNA, won't it?
4. Finally, is there a kind of amplification in the library construction that allows to assembly several reads in order to obtain a larger genomic sequence than those gathered by Minion (I mean larger than 16Kb, for instance).
Sorry if my questions annoyed anyone, but I am still as Illumina-thinker
Thank you for your help in advance.
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