I have measured the samples after the PCR and they had good measurement especially with gene B. After the beads some of them their concentration even increased. The PCR is a custom panel primers that are ready designed and have been tested before and worked very well.
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For example one of the samples was 52 ng/ul after PCR then for gene A after purification it changed to be 18,64 ng/ul. another sample was 22 ng/ul after AMPURe became 33,4 ng/ul however when I repeated the measurement now it is 8,92 after AmPure. The other problem is that the differences between the concentration in different samples is so variable between samples and with different genes. Example sample 9 is 17.38 ng/ul with gene A and 1.542 ng/ul in gene B. Could I still pool them? or run them in different chips?
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