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Old 10-09-2008, 01:16 PM   #1
genseq
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Default Mobious Biosystems: A new player in the third-gen space uncloaks....

Another third-gen sequencing company has posted some info. Thanks to genseq for reposting it here! See below for the announcement. -=ECO

****************

" Mobious Biosystems is proud to introduce the Nexus I, the worlds first and only 24hr Genome Sequencer. Drawing from over ten years experience in the field of next generation sequencing and employing unique biophysical & biochemical techniques the Nexus I offers unparalleled throughput and accuracy.

http://www.mobious.com/mobious_produ...18/page18.html

System Benefits:

Over 6 Gigabases in 24hrs
24hr unattended operation
Proprietary economically competitive reagents
Industry leading fragment read lengths
Remote system maintenance
Automated sample handling and fluidic procedures
Realtime loss-less data compression
Option to upgrade to the Nexus II system (60+ gigabases per day)
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Old 10-09-2008, 03:14 PM   #2
ScottC
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"For a polymerase to add a nucleotide to a growing DNA chain, it must first bind to the strand and then 'select' a nucleotide from solution in order to 'test' whether or not the nucleotide is a correct base or a 'match'. If a 'match' is confirmed, then the polymerase enzyme proceeds to add the base to the growing DNA molecule. If the base, however, turns out not to 'match' then it is returned to solution. These two sequences, match and mismatch, involve different periods. A match results in the base being added to the growing strand, which this involves several 'time consuming' steps and it is this time difference which is exploited in Mobious’s sequencing-by-synthesis approach. It places the polymerase in a flow, adds a single nucleotide at a time and derives sequence data by measuring the amount of time the polymerase attaches to the growing strand. A number of detection strategies may be applied to the technique. By simply labelling the polymerase with a fluorescent species, the approach can be applied in the context of conventional array formats. "

Sounds interesting. Is this an application of surface plasmon resonance?

Last edited by ScottC; 10-09-2008 at 03:21 PM.
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Old 10-09-2008, 03:41 PM   #3
ECO
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Very similar to PacBio by that description.
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Old 10-09-2008, 05:00 PM   #4
ScottC
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I think the difference, though, is that the Mobious technology uses a labelled polymerase, and unlabelled nucleotides. That seems to be the key benefit in their strategy and the reason for their claim to long read lengths. The polymerase is attached to solid phase which is also a sensing surface (the SPR part). So they have blocked nucleotides that can be unblocked by (perhaps) laser light, and they effectively measure the mass difference of the polymerase when the correct nucleotide is incorporated.

I should point out that that's not necessarily correct, but it's my understanding of their process!
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