Greetings!
I am getting ready to use the TruSeq RNA kit for the first time. I noticed the concentration of Ampure beads to sample decreases as you progress through the steps to prepare an RNASeq sample. I assume that as the bead concentration to DNA decreases, the maximum length of fragment washed away will increase.
Ex: 1.8:1 bead: DNA removes anything less than 100bp. Then perhaps 1:1 bead: DNA would remove anything less than 200bp.
1) Is this a correct assumption?
2) Has anyone found that varying the initial RNA concentration affects the maximum length of fragment washed away and results in a different final fragment length?
Thanks in advance for your help and sharing your knowledge on this preparation technique.
I am getting ready to use the TruSeq RNA kit for the first time. I noticed the concentration of Ampure beads to sample decreases as you progress through the steps to prepare an RNASeq sample. I assume that as the bead concentration to DNA decreases, the maximum length of fragment washed away will increase.
Ex: 1.8:1 bead: DNA removes anything less than 100bp. Then perhaps 1:1 bead: DNA would remove anything less than 200bp.
1) Is this a correct assumption?
2) Has anyone found that varying the initial RNA concentration affects the maximum length of fragment washed away and results in a different final fragment length?
Thanks in advance for your help and sharing your knowledge on this preparation technique.
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