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Comparison of Normalization Methods for Construction of Large Multiplex Amplicon Pools for Next-Generation Sequencing.
Appl Environ Microbiol. 2010 Apr 23;
Authors: Harris JK, Sahl JW, Castoe TA, Wagner BD, Pollock DD, Spear JR
Constructing mixtures of tagged or barcoded DNAs for sequencing is an important requirement for the efficient use of next-generation sequencers in applications where limited sequence data is required per sample. There are many applications in which next-generation sequencing can be effectively used to sequence large mixed samples; an example is the characterization of microbial communities where
Comparison of Normalization Methods for Construction of Large Multiplex Amplicon Pools for Next-Generation Sequencing.
Appl Environ Microbiol. 2010 Apr 23;
Authors: Harris JK, Sahl JW, Castoe TA, Wagner BD, Pollock DD, Spear JR
Constructing mixtures of tagged or barcoded DNAs for sequencing is an important requirement for the efficient use of next-generation sequencers in applications where limited sequence data is required per sample. There are many applications in which next-generation sequencing can be effectively used to sequence large mixed samples; an example is the characterization of microbial communities where