Hello,
Would love it if someone could clarify a couple of things concerning the tags in the SAM file from BLASR.
1) What do the YS and YE represent? Fairly sure they are start and end coordinates but, of what?
2) XQ is supposed to be the query sequence length, but where does it come from? I am trying to understand an inconsistency between the XQ value, and the subread coordinates. For example, how can a subread "movie/zmw/0_3904" have XQ:i:3984 ?
Thanks
Would love it if someone could clarify a couple of things concerning the tags in the SAM file from BLASR.
1) What do the YS and YE represent? Fairly sure they are start and end coordinates but, of what?
2) XQ is supposed to be the query sequence length, but where does it come from? I am trying to understand an inconsistency between the XQ value, and the subread coordinates. For example, how can a subread "movie/zmw/0_3904" have XQ:i:3984 ?
Thanks
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