SEQanswers

Go Back   SEQanswers > Literature Watch



Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Application of high-resolution, massively parallel pyrosequencing for estimat Newsbot! Literature Watch 0 09-21-2011 07:00 AM
PubMed: SICTIN: Rapid footprinting of massively parallel sequencing data. Newsbot! Literature Watch 0 08-17-2010 02:00 AM
PubMed: Gene discovery using massively parallel pyrosequencing to develop ESTs for th Newsbot! Literature Watch 0 05-21-2009 05:02 AM
PubMed: Multiplex HLA-Typing by Pyrosequencing. Newsbot! Literature Watch 0 10-08-2008 05:00 AM
PubMed: Accuracy and quality of massively parallel DNA pyrosequencing. Newsbot! Literature Watch 0 03-01-2008 05:40 AM

Reply
 
Thread Tools
Old 08-27-2009, 05:00 AM   #1
Newsbot!
RSS Posting Maniac
 

Join Date: Feb 2008
Posts: 1,439
Default PubMed: Rapid High-throughput HLA Typing by Massively-parallel Pyrosequencing for Hig

Syndicated from PubMed RSS Feeds

Related Articles Rapid High-throughput HLA Typing by Massively-parallel Pyrosequencing for High-Resolution Allele Identification.

Hum Immunol. 2009 Aug 22;

Authors: Gabriel C, Danzer M, Hackl C, Kopal G, Hufnagl P, Hofer K, Polin H, Stabentheiner S, Pröll J

Transplantation and notably haematopoietic stem cell transplantation require high resolution HLA typing and are therefore, because of the heterozygous genomic DNA samples, dependent on clonal analytical methods. High-resolution HLA typing is a necessity for accomplishing the best-possible histocompatibility match between donor and recipient, since mismatches strongly increase the risk of severe acute graft-versus-host disease. We describe the development and first application in a clinical setting of a novel, human leukocyte antigen sequence-based typing method by exploring the next-generation sequencing technology as provided by the Genome Sequencer FLX system (Roche / 454 Life Sciences, Branford, CT, USA). The developed system allows for ambiguity-free, high-throughput, high-resolution HLA-A and -B typing with the potential for automation. Primers and Genome Sequencer FLX specific adaptors were lengthened with donor-identifying barcode sequences to identify each of 8 Caucasian reference donors within one single multiplex sequencing run. Results, compared to normal SBT HLA typing show that with an average of 1000 reads per amplicon every patient was identified correctly. Furthermore, current investments for increased read lengths and fully automated molecular diagnostic software tools, using original GS-FLX data file formats, will enhance this novel HLA typing strategy in the near future.

PMID: 19706315 [PubMed - as supplied by publisher]



More...
Newsbot! is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 08:56 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO