Go Back   SEQanswers > Literature Watch

Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Analysing DNA methylation using bisulphite pyrosequencing. Newsbot! Literature Watch 0 09-14-2011 03:00 AM
PubMed: Validation of two ribosomal RNA removal methods for microbial metatranscripto Newsbot! Literature Watch 3 10-04-2010 07:44 AM
PubMed: 16SrRNA bacterial tag-encoded amplicons pyrosequencing for rapid deciphering Newsbot! Literature Watch 0 05-05-2009 06:00 AM
PubMed: Bacterial Tag-Encoded FLX Amplicon Pyrosequencing (bTEFAP) for Microbiome Stu Newsbot! Literature Watch 0 08-21-2008 06:00 AM

Thread Tools
Old 12-24-2010, 03:13 AM   #1
RSS Posting Maniac

Join Date: Feb 2008
Posts: 1,443
Default PubMed: Ribosomal tag pyrosequencing of DNA and RNA from benthic coral reef microbiot

Syndicated from PubMed RSS Feeds

Ribosomal tag pyrosequencing of DNA and RNA from benthic coral reef microbiota: community spatial structure, rare members and nitrogen-cycling guilds.

Environ Microbiol. 2010 Dec 22;

Authors: Gaidos E, Rusch A, Ilardo M

Ribosomal tag libraries based on DNA and RNA in coral reef sediment from Hawaii show the microbial community to be dominated by the bacterial phyla Proteobacteria, Firmicutes and Actinobacteria, the archaeal order Nitrosopumilales and the uncultivated divisions Marine Group III (Euryarchaeota) and Marine Benthic Group C (Crenarchaeota). Operational taxonomic units (OTUs) number in the high thousands, and richness varies with site, presence or absence of porewater sulfide (sediment depth), and nucleotide pool. Rank abundance curves of DNA-based libraries, but not RNA-based libraries, possess a tail of low abundance taxa, supporting the existence of an inactive 'rare' biosphere. While bacterial libraries from two oxic samples differ markedly, those from two anoxic (sulfidic) samples are similar. The four dominant bacterial OTUs are members of genera that include pathogens, but are found in marine environments, and include facultative anaerobes, i.e. dissimilatory nitrate reducers and denitrifiers. This may explain their abundance in both oxic and anoxic samples. A numerous archaeon is closely related to the lithoautotrophic ammonia oxidizer Nitrosopumilus maritimus. Known bacterial ammonia oxidizers are essentially absent, but bacterial nitrite oxidizers are abundant. Although other studies of this reef found evidence for anaerobic ammonia oxidizers, primer bias rendered that clade invisible to this study.

PMID: 21176054 [PubMed - as supplied by publisher]

Newsbot! is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 03:46 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO