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Old 03-26-2019, 04:23 AM   #1
ChiaraGen
Junior Member
 
Location: Italy

Join Date: Mar 2019
Posts: 1
Default Haloplex HS method

Hello!
I have two questions about Haloplex HS protocol:

1. I read that Haloplex uses double stranded byotinilated probes and during hybridization step I put with probes also indexes. Someone Know's how the indexes hybridize with the target and probes creating the ring? Beacuse I know that the probes contain a sequence complementary to my target and a site where there are sequencing primer motif, index,adapters, pcr primers and molecular barcode. Where the indexes hybridize?

2.Why Haloplex is defined as an amplicon based method while SureSelect a capure method? (see the paper "Evaluation of Hybridization Capture Versus
Amplicon-Based Methods for Whole-Exome Sequencing"2015)
Both methods use PCR but in different moment, so I don't understand... and also I don't understand why capture method demonstrate better uniformity.

Thank you very much!
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