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Old 03-27-2019, 11:56 PM   #1
Gurs
Junior Member
 
Location: israel

Join Date: Mar 2019
Posts: 2
Default running RTA offline

Hi,
After some data of HiSeq2500 run were accidentally deleted, I have the following files for each tile in the 'Processed' folder:
*.errorMap
*.qms
*.stats
*.FWHMMap
*.align
*.bqm
*.readErrors
*.bqm
*.clocs
*.control
*.ctr
*.filter
*.tempdensity
But I don't have *.bcl or *.cif files.
Is there any option to create bcl files for this run? My RTA version is 1.18.66.4. I saw that there is an option to run RTA offline (OLB, off-line base-calling), but when I run it (double-click the RTAstart.bat file) I get the message that bcl files are already generated.
Thanks for helping.
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Old 04-02-2019, 09:07 AM   #2
misterc
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Location: Livermore, CA

Join Date: Jan 2016
Posts: 42
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No, without .bcl or .cif files you're stuck.
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