Hey everyone,
So i've been trying to make libraries from low-ish input (i.e.: 25-50ng total RNA) using Linearized DNA Amplification and NEB Next Ultra. RNA was polyA selected and fragmented. I'm getting this weird sharp peak at ~330 bp (see attached) after amplification (19 cycles) and I'm not quite sure what to make of it. Looking for any thoughts or suggestions. If you need more details let me know.
Thanks!
Giacomo
So i've been trying to make libraries from low-ish input (i.e.: 25-50ng total RNA) using Linearized DNA Amplification and NEB Next Ultra. RNA was polyA selected and fragmented. I'm getting this weird sharp peak at ~330 bp (see attached) after amplification (19 cycles) and I'm not quite sure what to make of it. Looking for any thoughts or suggestions. If you need more details let me know.
Thanks!
Giacomo
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