Hi,
I got 112GB raw data from nextera rapid capture enrichment of nextseq500. FastQ files are approximately 7-9 GB. When I try to annotate variants, the number of variants are nearly 200.000, which is quite low for an exome sequencing result. It is like a bioinformatic error that cannot interpret the variants from the data. Or could it be caused by something else?
Two samples- both have 8GB FASTQ- one has 200.000 variants, other has 1.000.000 variants. How could it be possible ?
Thanks
I got 112GB raw data from nextera rapid capture enrichment of nextseq500. FastQ files are approximately 7-9 GB. When I try to annotate variants, the number of variants are nearly 200.000, which is quite low for an exome sequencing result. It is like a bioinformatic error that cannot interpret the variants from the data. Or could it be caused by something else?
Two samples- both have 8GB FASTQ- one has 200.000 variants, other has 1.000.000 variants. How could it be possible ?
Thanks
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