Hi folks
I am planning to use illumina primers RP1 and RPI1 with phosphorothioate bonds between the last base and the penultimate base at the 3' end. So my eventual PCR library will contain two phoshorothioate bonds, one on each strand.
Can polymerase read through such products during cluster formation or regular PCR?
Thanks in advance.
BB
I am planning to use illumina primers RP1 and RPI1 with phosphorothioate bonds between the last base and the penultimate base at the 3' end. So my eventual PCR library will contain two phoshorothioate bonds, one on each strand.
Can polymerase read through such products during cluster formation or regular PCR?
Thanks in advance.
BB
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