Hi all
I have done few chips using antibody against a transcription factor in honeybee. I also used pre immune IgG (from the animal where antibody was generated) and did chip in identical way. I have now used input as control and have found peaks in both the experimental chip data and the control (chip using pre immune IgG ) data.
Some of the peaks found in experimental dataset are also turning up in control dataset. However, the fold enrichment differs- say if the experimental dataset shows an enrichment of 100 fold, the control dataset shows enrichment of 10 fold.
To get the final data- should i subtract the control dataset from experimental in a quantitative manner or just qualitative way. I mean should I consider the fold enrichment while subtraction or should I just ignore it
Thanks
naveen
I have done few chips using antibody against a transcription factor in honeybee. I also used pre immune IgG (from the animal where antibody was generated) and did chip in identical way. I have now used input as control and have found peaks in both the experimental chip data and the control (chip using pre immune IgG ) data.
Some of the peaks found in experimental dataset are also turning up in control dataset. However, the fold enrichment differs- say if the experimental dataset shows an enrichment of 100 fold, the control dataset shows enrichment of 10 fold.
To get the final data- should i subtract the control dataset from experimental in a quantitative manner or just qualitative way. I mean should I consider the fold enrichment while subtraction or should I just ignore it
Thanks
naveen
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