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Old 06-18-2018, 01:31 PM   #1
Location: U.S.A

Join Date: Nov 2013
Posts: 23
Default RNA-seq analysis from low biomass samples

I have access to a sample that we hope to run a microbial ecological study on. The problem is that each sample only has about one picogram of DNA.

What are the best molecular biology and/or bioinformatics techniques to look into to maximize the chance I can develop RNA-sequencing analysis techniques from such low biomass samples?

Thank you for sharing any advice!

Last edited by SuzuBell; 06-18-2018 at 01:46 PM.
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Old 06-18-2018, 11:55 PM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,248

I do not think that there is any hyper sensitive method yet to preprare RNA-Seq library from 1 pg RNA.
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