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  • sfffile converting quality values

    Hi,

    has anyone experience with the -r option of the Roche tool sfffile ? According to the manual this convertes old quality values into Phred-like values using a quality lookup table.

    Well, I tried to convert old GS20 quality values and the tool fails to find the table:

    "The rescore option was specified, but cannot find rescore config. file (quality score lookup table)."

    I think I found the tables in the config directory of the Roche software (qualityScoreLookup_gs20.dat) but I don't know how to tell the tool where to look for this table.

    Has anyone an idea ?

    Thanks,

    Andreas

  • #2
    According to the technical bulletin accompanying patch 2.0.0.20-1 this was due to a bug in the original release of the v2.0 software. Installing the patch should resolve the issue.

    Comment


    • #3
      Patching solved the problem ...

      Thanks,

      Andreas

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