Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • CanFam2 data RNAseq samples and GenomicRanges

    hi All,
    I am processing RNAseq samples for canFam2. I used the following commands from GenomicRanges package that gives me only 1218 genes. (because by default refGene for canFam2 retains only known genes.)

    txdb <- makeTranscriptDbFromUCSC(genome = "canFam2", tablename = "refGene")
    TranscriptDb object:
    | Db type: TranscriptDb
    | Data source: UCSC
    | Genome: canFam2
    | UCSC Table: refGene
    | Type of Gene ID: Entrez Gene ID
    | Full dataset: yes
    | transcript_nrow: 1218
    | exon_nrow: 11256
    | cds_nrow: 10922
    | Db created by: GenomicFeatures package from Bioconductor
    | Creation time: 2011-08-08 12:56:52 -0400 (Mon, 08 Aug 2011)
    | GenomicFeatures version at creation time: 1.4.2
    | RSQLite version at creation time: 0.9-4
    | DBSCHEMAVERSION: 1.0

    To get all the genes, I changed the table name to "xenoRefGene" but that does not fetch the "gene Ids". Also, the number of transcripts (229403) looks abnormally large.

    TranscriptDb object:
    | Db type: TranscriptDb
    | Data source: UCSC
    | Genome: canFam2
    | UCSC Table: xenoRefGene
    | Type of Gene ID: no gene ids
    | Full dataset: yes
    | transcript_nrow: 229403
    | exon_nrow: 1110585
    | cds_nrow: 747878
    | Db created by: GenomicFeatures package from Bioconductor
    | Creation time: 2011-08-08 12:51:29 -0400 (Mon, 08 Aug 2011)
    | GenomicFeatures version at creation time: 1.4.2
    | RSQLite version at creation time: 0.9-4
    | DBSCHEMAVERSION: 1.0


    please help.

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Yesterday, 08:47 AM
0 responses
13 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
60 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
60 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
54 views
0 likes
Last Post seqadmin  
Working...
X