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I am a biologist and a poor programmer. I have assembled RNA-seq data from which I'd like to extract all potential long ncRNA candidates. I assume the easiest way would be to compare my data to Refseq or Rfam but I don't know how to do this. Furthermore, this strategy would only pick up already annotated lncRNA. I would be glad of any help.
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Last edited by stephenhart; 09-28-2011, 11:45 PM. -
You can use Dario web server which is a free web tool for ncRNA analysis using RNAseq data.
I have not used it before. so can not comment on the results. Please let me know your experience wit it. -
You could do it with Annocript. It annotates the transcriptome and gives putative lnc RNAs sequences...Comment
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