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Old 11-05-2013, 01:39 PM   #1
gwilymh
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Location: Milwaukee

Join Date: Dec 2011
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Default Evaluating overdispersion in CummeRbund

Hi,

I am analyzing RNA-Seq data in a TopHat/Cufflinks/CummeRbund pipeline. In the CummeRbund manual, it is recommended that researchers check for overdispersion for each sample as a quality control. How does one tell if a sample is overdispersed? Is there a statistical test that can quantify overdispersion?

I have attached some of my dispersion plots for gene expression. Is there any way to tell if they are overdispersed?
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File Type: pdf dispersionPlots.pdf (2.27 MB, 50 views)
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