Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
How much starting material? hambone 454 Pyrosequencing 1 01-20-2012 06:38 AM
amplication free whole genome seq with small amounts of starting material. kingsalex Sample Prep / Library Generation 3 01-20-2011 05:43 AM
Can I use sheared genomic DNA as starting material of bacterial genome sequencing? omnivore 454 Pyrosequencing 6 10-13-2010 06:11 AM
small amount of DNA material for Helicos? danf1985 Helicos / Direct Genomics 1 02-19-2010 11:50 AM
concentration of my starting material? seqgirl123 Illumina/Solexa 6 07-28-2009 07:19 PM

Thread Tools
Old 01-04-2012, 04:36 PM   #1
Junior Member
Location: New York

Join Date: Dec 2011
Posts: 2
Default amount of starting material for a 454 run

I am planning to deep sequence material from 13 samples. For this, I am isolating RNA from various organs, reverse transcribing with a gene specific primer and performing a multiplex PCR (all products are ~400 bp). Then, I will ligate a barcode to the PCR product from each sample and sequence. Since I am jumping into the middle of the standard 454 prep, can someone please tell me how much starting material I need for each sample for a full 454 plate?
hambone is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 08:59 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO