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Old 05-22-2019, 08:13 AM   #1
BexMultiplex
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Default Nextera DNA Flex Library Prep - TWB

Does anyone know the composition of the Tagment Wash Buffer in the Nextera DNA Flex Library Prep (20018704) protocol? Does it contain alcohol? PEG?

TIA
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Old 05-22-2019, 04:52 PM   #2
luc
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I would check out the SMART-SEQ2 protocol. They did homebrew something similar. I vaguely remember SDS to denature the enzyme.
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Old 05-29-2019, 11:32 AM   #3
UCan'tBcereus
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Hi BexMultiplex,

According to the Nextera Flex patent, TWB is:

-100 mM Tris HCl pH 8.5
-100 mM NaCL
-0.1% Tween 20

https://patents.google.com/patent/US...ed+transposome

We have a Tecan, and are going to be reducing RXN volumes down to 1/4, and at that point TWB becomes a limiting reagent. I will be trying my own eventually, but I have not used it yet personally myself, so i can't promise it will work

If you are ever trying to find what a reagent is, looking at the patent (if you can find it) is probably the best way to get this kind of information. It also really helpful for understanding how a kit work when the protocol is vague.

Hope this helps!
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Old 05-30-2019, 07:19 AM   #4
BexMultiplex
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Thanks @UCan'tBcereus, that's really helpful!
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