Hello everyone,
We've been having problems with our base calls, especially A and T, diverging over the course of a run (see attached screen shot). The runs are whole genome sequencing runs of bacterial genomes using Nextera XT, v3, 2 x 300 PE.
Obviously this results in all sorts of error generation, and we're having difficulty figuring out what's happening. We've spoken with tech support and so far haven't heard anything, and have spoken with several other people at Illumina as well who also haven't been able to help. Any thoughts on what could be causing this and what to do about it?
thanks!
We've been having problems with our base calls, especially A and T, diverging over the course of a run (see attached screen shot). The runs are whole genome sequencing runs of bacterial genomes using Nextera XT, v3, 2 x 300 PE.
Obviously this results in all sorts of error generation, and we're having difficulty figuring out what's happening. We've spoken with tech support and so far haven't heard anything, and have spoken with several other people at Illumina as well who also haven't been able to help. Any thoughts on what could be causing this and what to do about it?
thanks!
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