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08-11-2011, 06:20 AM
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#1 |
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Member
Location: Baltimore Join Date: Mar 2010
Posts: 38
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Questions about Scriputre RPKM
Hi.
I only have two RNA-Seq samples. Could I directly use RPKM ratio to find out differentially expressed genes between these two samples? RPKM values were generated by Scripture. Wish your help! Thanks a bunch! |
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08-12-2011, 06:10 AM
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#2 |
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Member
Location: Beijing Join Date: Jul 2011
Posts: 74
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Yes, RPKM was designed to normalize gene depth, read number.. but the ratio is somehow arbitary, without significant level.
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