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Old 08-11-2011, 06:20 AM   #1
Location: Baltimore

Join Date: Mar 2010
Posts: 38
Question Questions about Scriputre RPKM


I only have two RNA-Seq samples. Could I directly use RPKM ratio to find out differentially expressed genes between these two samples? RPKM values were generated by Scripture.

Wish your help! Thanks a bunch!
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Old 08-12-2011, 06:10 AM   #2
Location: Beijing

Join Date: Jul 2011
Posts: 74

Yes, RPKM was designed to normalize gene depth, read number.. but the ratio is somehow arbitary, without significant level.
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