SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Pippin Prep as a service? darthsequencer Core Facilities 0 07-30-2013 02:36 PM
eGel, Qubit, Bioanalyzer: when and why? Nu2NGS Sample Prep / Library Generation 6 06-22-2012 02:54 AM
Pippin Prep for RNA? SageSigh Sample Prep / Library Generation 8 07-23-2011 08:41 AM
Fragment Size shift on Egel bye using the new TrueSeq protocol ? Claire1 Illumina/Solexa 7 05-11-2011 08:01 AM
Fragment Size shift on Egel bye using the new TruSeq protocol ? Claire1 Sample Prep / Library Generation 2 05-05-2011 01:37 PM

Reply
 
Thread Tools
Old 03-05-2014, 01:21 PM   #1
kwalton
Junior Member
 
Location: Denver

Join Date: Jan 2014
Posts: 5
Default Pippin vs. Egel

Hi all,
We are looking for opinions on whether you prefer the Pippin or E gel for size selection when doing NGS libraries. Has anyone compared them side by side? We are a low throughput lab with the opportunity to become high throughput in the near future. We are working on quite a few protocols with one being a custom protocol that needs 3 separate gel extractions. Currently those are being done on agarose gels, with only 1 sample/gel each time this step is needed. We need something more high throughput than that and would also like to avoid the cleanup step after agarose gel extraction. Any opinions, pros or cons on either machine would be greatly appreciated.
Thanks
kwalton is offline   Reply With Quote
Old 03-05-2014, 01:43 PM   #2
kwaraska
Senior Member
 
Location: Boston,MA

Join Date: Nov 2008
Posts: 122
Default

The egel is more hands on. The pippin prep takes longer but you can set it to do what you need it to and walk away.
kwaraska is offline   Reply With Quote
Reply

Tags
e-gel, pippin

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 07:25 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO