Pippin vs. Egel

kwalton · 03-05-2014, 01:21 PM

Hi all,
We are looking for opinions on whether you prefer the Pippin or E gel for size selection when doing NGS libraries. Has anyone compared them side by side? We are a low throughput lab with the opportunity to become high throughput in the near future. We are working on quite a few protocols with one being a custom protocol that needs 3 separate gel extractions. Currently those are being done on agarose gels, with only 1 sample/gel each time this step is needed. We need something more high throughput than that and would also like to avoid the cleanup step after agarose gel extraction. Any opinions, pros or cons on either machine would be greatly appreciated.
Thanks

kwaraska · 03-05-2014, 01:43 PM

The egel is more hands on. The pippin prep takes longer but you can set it to do what you need it to and walk away.

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03-05-2014, 01:21 PM	#1
kwalton Junior Member Location: Denver Join Date: Jan 2014 Posts: 5	Pippin vs. Egel Hi all, We are looking for opinions on whether you prefer the Pippin or E gel for size selection when doing NGS libraries. Has anyone compared them side by side? We are a low throughput lab with the opportunity to become high throughput in the near future. We are working on quite a few protocols with one being a custom protocol that needs 3 separate gel extractions. Currently those are being done on agarose gels, with only 1 sample/gel each time this step is needed. We need something more high throughput than that and would also like to avoid the cleanup step after agarose gel extraction. Any opinions, pros or cons on either machine would be greatly appreciated. Thanks

03-05-2014, 01:43 PM	#2
kwaraska Senior Member Location: Boston,MA Join Date: Nov 2008 Posts: 122	The egel is more hands on. The pippin prep takes longer but you can set it to do what you need it to and walk away.