Hi,
I am attempting the Library Quantitation phase of the Rapid Library Prep. set up
ie. Producing a standard curve of fluorescence through 8 serial dilutions of RL
Standard.
However the fluorometer I am using (Invitrogen Qubit 2.0) does not seem to give the option of giving results in RFU.
So....I am able to measure the concentration of my sample in ng/uL.
BUT....the 'Rapid Library Calculator' only accepts measurements in RFU.
AND...I need to ultimately calculate the concentration of my library in molecules/uL.
Is there a way to work around this that anybody knows of?
I am attempting the Library Quantitation phase of the Rapid Library Prep. set up
ie. Producing a standard curve of fluorescence through 8 serial dilutions of RL
Standard.
However the fluorometer I am using (Invitrogen Qubit 2.0) does not seem to give the option of giving results in RFU.
So....I am able to measure the concentration of my sample in ng/uL.
BUT....the 'Rapid Library Calculator' only accepts measurements in RFU.
AND...I need to ultimately calculate the concentration of my library in molecules/uL.
Is there a way to work around this that anybody knows of?
Comment