I'm about to start a project where I want to pool all existing transcriptomes in SRA for a nonmodel species without reference genome to study differentially expressed genes and the kind. Might worth mentioning that the transcriptomes I want to pool mostly come from different tissues.
Have you ever done something similar? Is there anything I should be extra careful about (batch effect, ...)? Do you have some literature suggestion about something similar to my project? I only found papers about merging microarray data until now.
Have you ever done something similar? Is there anything I should be extra careful about (batch effect, ...)? Do you have some literature suggestion about something similar to my project? I only found papers about merging microarray data until now.
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