The submission that will never die...
I have a number of contigs that did not pass NCBI's contamination/adapter screen. I need to trim these but the problem is that they all are varying lengths. Some internal bust most at either the 5' or 3' end of the scaffold.
The only info provided by NCBI is the scaffold/contig name, length and the start and stop base # of what needs to be trimmed. If I had the sequence I could just manually ctrl-f and delete/substitute Ns.
Most of these are too large to load into VectorNTI or similar program.
Any help would be appreciated. Thanks.
I have a number of contigs that did not pass NCBI's contamination/adapter screen. I need to trim these but the problem is that they all are varying lengths. Some internal bust most at either the 5' or 3' end of the scaffold.
The only info provided by NCBI is the scaffold/contig name, length and the start and stop base # of what needs to be trimmed. If I had the sequence I could just manually ctrl-f and delete/substitute Ns.
Most of these are too large to load into VectorNTI or similar program.
Any help would be appreciated. Thanks.
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