Hi all,
I got less than 10% reads in unmapped.bam by tophat (Arabidopsis RNA-seq).
Then I took some of them and did blast on NCBI. I expected to see those reads would align to some other species, however, all the reads I tried were still Arabidopsis mRNA. This confused me! Anyone has clue for it?
THANKS!
I got less than 10% reads in unmapped.bam by tophat (Arabidopsis RNA-seq).
Then I took some of them and did blast on NCBI. I expected to see those reads would align to some other species, however, all the reads I tried were still Arabidopsis mRNA. This confused me! Anyone has clue for it?
THANKS!
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